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novoalign version 3.02.13  (Thermo Fisher)


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    Structured Review

    Thermo Fisher novoalign version 3.02.13
    Novoalign Version 3.02.13, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/novoalign/pm39806575-34-6-17
    Average 90 stars, based on 1 article reviews
    novoalign version 3.02.13 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Leukemia relapse via genetic immune escape after allogeneic hematopoietic cell transplantation
    Article Snippet: .. Details of the bioinformatic approach to investigate somatic HLA mutational status have been described elsewhere.14 In brief, after obtaining a confident full 4th field typing through NovoHLA typing algorithm (Novocraft Technologies), paired-end reads from either targeting sequencing were directly aligned on a per-patient HLA reference using Novoalign (Novocraft Technologies Sdn Bhd). ..

    Article Title: Prevalence of TTR variants detected by whole-exome sequencing in hypertrophic cardiomyopathy.
    Article Snippet: Exome sequencing of the enriched libraries occurred on the Illumina Hiseq4000 platform at a minimum of 30 coverage, using standard manufacturer’s protocols. .. Sequence reads alignment against the reference human genome (UCSC hg19) was performed using NovoAlign (Novocraft Technologies Sdn Bhd). .. Picard (http://picard. sourceforge.net/) was used for duplicate reads removal, format conversion, and indexing.

    Article Title: Leukemia relapse via genetic immune escape after allogeneic hematopoietic cell transplantation
    Article Snippet: Severity of acute GvHD was graded according to Glucksberg’s criteria5 whereas the assessment of all patients developing chronic GVHD was made according to the National Institute of Health (NIH) consensus criteria.6,7 DNA isolation Genomic DNA was isolated directly from cryopreserved unfractionated peripheral or bone marrow blood mononuclear cells with the Nuclei Lysis Solution (Promega) according to manufacturer’s instructions. .. HLA mutational analysis Details of the bioinformatic approach to investigate somatic HLA mutational status have been described elsewhere.8 In brief, after obtaining a confident full 4th field typing through NovoHLA typing algorithm (Novocraft Technologies), paired-end reads from either targeting sequencing were directly aligned on a per-patient HLA reference using Novoalign (Novocraft Technologies Sdn Bhd). ..

    Software:

    Article Title: Verification of Underlying Genetic Cause in a Cohort of Russian Patients with Familial Hypercholesterolemia Using Targeted Next Generation Sequencing
    Article Snippet: Sequencing was performed using the Illumina HiSeq 1500 platform (Illumina, San Diego, CA, USA) with 2 × 100 base pair read length following Illumina protocols. .. Bioinformatics analysis was performed by means of a custom pipeline that includes software as NovoAlign (Novocraft Technologies Sdn Bhd), SAMtools and BCFtools (Sanger Institute) for variant calling and genotyping and Annovar for variant annotation. ..

    Variant Assay:

    Article Title: Verification of Underlying Genetic Cause in a Cohort of Russian Patients with Familial Hypercholesterolemia Using Targeted Next Generation Sequencing
    Article Snippet: Sequencing was performed using the Illumina HiSeq 1500 platform (Illumina, San Diego, CA, USA) with 2 × 100 base pair read length following Illumina protocols. .. Bioinformatics analysis was performed by means of a custom pipeline that includes software as NovoAlign (Novocraft Technologies Sdn Bhd), SAMtools and BCFtools (Sanger Institute) for variant calling and genotyping and Annovar for variant annotation. ..



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    Illumina Inc giab ashkenazim trio hg002 and hg004 illumina 2x250bp novoalign grch38 bam files
    Performance benchmark results of reimplemented flagstats versus stock version. (A) Results using the <t>GIAB</t> <t>HG002</t> Illumina 2x250 BAM file with an NVMe SSD array available on AWS. (B) Results using the rapid autopsy Bn2 sample BAM file with an NVMe SSD array available on AWS. (C) Results using the GIAB HG002 Illumina 2x250 BAM file with a Lustre distributed file system. (D) Results using the rapid autopsy Bn2 sample BAM file with a Lustre distributed file system.
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    Image Search Results


    Performance benchmark results of reimplemented flagstats versus stock version. (A) Results using the GIAB HG002 Illumina 2x250 BAM file with an NVMe SSD array available on AWS. (B) Results using the rapid autopsy Bn2 sample BAM file with an NVMe SSD array available on AWS. (C) Results using the GIAB HG002 Illumina 2x250 BAM file with a Lustre distributed file system. (D) Results using the rapid autopsy Bn2 sample BAM file with a Lustre distributed file system.

    Journal: Bioinformatics

    Article Title: quickBAM: a parallelized BAM file access API for high-throughput sequence analysis informatics

    doi: 10.1093/bioinformatics/btad463

    Figure Lengend Snippet: Performance benchmark results of reimplemented flagstats versus stock version. (A) Results using the GIAB HG002 Illumina 2x250 BAM file with an NVMe SSD array available on AWS. (B) Results using the rapid autopsy Bn2 sample BAM file with an NVMe SSD array available on AWS. (C) Results using the GIAB HG002 Illumina 2x250 BAM file with a Lustre distributed file system. (D) Results using the rapid autopsy Bn2 sample BAM file with a Lustre distributed file system.

    Article Snippet: GIAB Ashkenazim Trio HG002 and HG004 Illumina 2x250bp novoalign GRCh38 BAM files are available at The rapid autopsy tumor normal sample dataset was from a published study ( ).

    Techniques:

    Performance benchmark results of reimplemented snp-pileup versus stock version. Note that the stock implementation of snp-pileup does not support multi-threading. (A) Results using the GIAB HG002 and HG004 Illumina 2x250 BAM files with an NVMe SSD array available on AWS. (B) Results using the rapid autopsy Bn2 and Germ1 BAM files with an NVMe SSD array available on AWS. (C) Results using the GIAB HG002 and HG004 Illumina 2x250 BAM file with a Lustre distributed file system. (D) Results using the rapid autopsy Bn2 and Germ1 BAM file with a Lustre distributed file system.

    Journal: Bioinformatics

    Article Title: quickBAM: a parallelized BAM file access API for high-throughput sequence analysis informatics

    doi: 10.1093/bioinformatics/btad463

    Figure Lengend Snippet: Performance benchmark results of reimplemented snp-pileup versus stock version. Note that the stock implementation of snp-pileup does not support multi-threading. (A) Results using the GIAB HG002 and HG004 Illumina 2x250 BAM files with an NVMe SSD array available on AWS. (B) Results using the rapid autopsy Bn2 and Germ1 BAM files with an NVMe SSD array available on AWS. (C) Results using the GIAB HG002 and HG004 Illumina 2x250 BAM file with a Lustre distributed file system. (D) Results using the rapid autopsy Bn2 and Germ1 BAM file with a Lustre distributed file system.

    Article Snippet: GIAB Ashkenazim Trio HG002 and HG004 Illumina 2x250bp novoalign GRCh38 BAM files are available at The rapid autopsy tumor normal sample dataset was from a published study ( ).

    Techniques: